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Research Registry No |
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HRID-00104_V11 |
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University Departmental Research |
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Registration Date |
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2019-05-30 |
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Title of Research |
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Amino acid sequence determination of phospholipase A2 from Russell’s viper
(Daboia siamensis) venom in Myanmar |
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Principle Investigator |
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Thet Thet Mar |
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Co-authors |
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Zaw Myint, Moe Thida Kyaw, Nwe Nwe Oo, Lwin Zar Maw, Win Aung |
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Field of Research |
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Biochemistry |
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Publication Source |
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Myanmar Health s Research Congress. 2015 |
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Year of Publication |
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2015 |
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URL of Publication |
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www.mhrc-mohs.com |
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Presentation Source |
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43rd Myanmar Health Research Congress. 2015 |
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Placement of Presentation |
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Department of Medical Research, Yangon, Myanmar |
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Year of Presentation |
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2015 |
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Abstract |
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Phospholipase A2 (PLA2) (EC3.1.1.4) enzyme which is a major component of snake venoms and commonly found in all snake venoms and has various biological effects including neurotoxicity, cardiotoxicity, myotoxicity, coagulation and hypotension. The aim of this study is to determine the amino acid sequence of PLA2 from Myanmar Russell’s viper venom which will be useful in construction of primer design and subsequent cloning of PLA2 enzyme from viper venom gland. The PLA2 enzyme was purified by using conventional Sephadex G-75 gel filtration chromatography and DEAE Sepharose ion exchange chromatography. Its purity was confirmed by SDS-PAGE and activity was measured by using Marinetty method. Then the amino acid sequence of purified PLA2 was determined by the amino acid sequencing machine (Shimadzu PPSQ 31A) at Department of Medical Research (Lower Myanmar). The sequence obtained was aligned with other PLA2 toxins from the online BLAST (Basic Local Alignment Search Tool) program of the National Center for Biotechnology Information. It showed that the first 10 N-terminal amino acid sequence (NLFEFAEMIV; AsnLeuPheGluPheAlaGluMetIleVal) was highly homologous with those of the PLA2s from the venoms of Daboia russelii russelii (Indian Russell’s viper), and Viperia russelii formosensis (Taiwan Russell’s viper). In conclusion, the amino acid sequence determination has been successfully established in Myanmar for the first time and the PLA2 sequence can be used for cDNA expression in an appropriate vector for developing venom-toxin specific antibodies which have significant impact on snake bite management. |
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IRB/PRC/ERC Approval Date |
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2014-09-17 |
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Placement of IRB/PRC/ERC |
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Department of Medical Research (Lower Myanmar) |
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IRB/PRC/ERC Approval Letter/Document |
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PLA2 sequence.pdf
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Pre-existing Registration ID |
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- |
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Pre-existing Name of Organization |
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Pre-existing Website |
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