Health Research Registry List

Research Registry No : HRID-00104_V3
University Departmental Research :
Registration Date : 2019-05-29
Title of Research : Determination aflatoxin B1 in betel nut (Kun Thee) Areca catechu Linn.
Principle Investigator : Thet Thet Mar
Co-authors : Thaung Hla, Myo Myo Kyaw, Zin Mei thein, Wai Lwin Oo
Field of Research : Others
Publication Source : 43rd Myamar Health Research Congress. 2015; P 97
Year of Publication : 2015
URL of Publication :
Presentation Source : 43rd Myanmar Health Research Congress. 2015
Placement of Presentation : Department of Medical Research, Yangon, Myanmar
Year of Presentation : 2015
Abstract : Areca nut is main component of betel quid and enjoyed in South and Southeast Asia. Aflatoxin B1 (AFB1) is the naturally occurring secondary metabolite and most potent hepatic carcinogen and mutagen produced by certain species of Aspergillus flavus, Aspergillus parasiticus. Objective: To determine the AFB1 level in areca nuts and notify the betel quid chewers about the danger of AFB1 contamination in areca nuts. Materials and Methods: Total of 42 areca nut samples were collected from 26 betel quid shops, five retailers and 11 markets in Yangon region. ELISA method was used to determine the level of AFB1 in tested samples. Results: All samples were contaminated with AFB1 in this study. The detected levels of AFB1 were 7.05-21.58ppb in 26 samples (61.9%), 5.18-21.66ppb in 5 samples (11.9%) and 5.48-21.08ppb in 11 samples (26.2%) respectively. AFB1 level of all samples was more than maximum permissible level of 5ppb according to the European Commission. Conclusions: This study highlighted that AFB1 was contaminated in areca nuts and to promote awareness for presence AFB1 in areca nut among the betel quids or area nut chewers. Betel quid and areca nut chewing are not only major risk factor of oral cancer but also chronic exposure of AFB1 at low levels lead to a high risk of developing cancer and low resistance to infectious diseases. Recommendation: Regular monitoring for contamination of AFB1 in areca nut should be promoted. The education to use rapid test kits for AFB1 determination should be given to betel quid sellers.
IRB/PRC/ERC Approval Date : 2014-02-17
Placement of IRB/PRC/ERC : Department of Medical Research (Lower Myanmar)
IRB/PRC/ERC Approval Letter/Document : AFB1.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -