Health Research Registry List

Research Registry No : HRID-00104_V4
University Departmental Research :
Registration Date : 2019-05-30
Title of Research : Characterization of biochemical composition and biological activities of Cobra (Naja kaouthia) venom
Principle Investigator : Thet Thet Mar
Co-authors : Win Aung, Zaw Myint, Tin Ko Ko Oo, Lwin Zar Maw
Field of Research : Biochemistry
Publication Source : Myanmar Health Research Congress, 2018
Year of Publication : 2018
URL of Publication :
Presentation Source : 46th Myanmar Health Research Congress. 2018; P95
Placement of Presentation : Department of Medical Research, Yangon, Myanmar
Year of Presentation : 2018
Abstract : Second common species of snake, responsible for high mortality rate in Myanmar is Cobra(Naja kaouthia). Cobra venom contains several enzymes and proteins including cobra venom factor, kaouthiagin, phospholipases, cardiotoxin, neurotoxin and myotoxin. This study was conducted to determine the biochemical compositions and biological activities of cobra venom. Crude venom was fractionated by using HiTrapFF cation exchange chromatography into seven protein peaks. Characterization of crude venom on SDS-PAGE revealed that proteins were found to have molecular mass ranged between 5 and 250kDa. The enzymatic activity of phospholipase A2(PLA2) was found in fraction 1,2,3,6 and 7. Activities of hyaluronidase, protease, acetylthiocholine, alkaline phosphomonoesterase and phosphodiesterase were detected in fraction 3, 3, 6, 2 and 6, 4 and 6 respectively. The LD50(i.v) of venom was 0.225mg/kg and above LD50 dose injected mice died within 2hrs. Neurotoxic symptoms like difficulty in movement and breathing were observed after injection of LD50 dose and some mice died within 1 hr. The concentration of serum CK from heart vessel after injection of LD100 of venom in mouse was 858.97U/L. When citrated plasma was treated with 0.2 µg each protein from all fractions, no clot was found till 25mins. Similarly when citrated plasma was treated with 0.01, 0.1 and 0.5 µg of crude venom, no clot formation occurs till 25 minutes. When detected with cobra ASV(1:100dilution) by ELISA method, positive signals could be detected in venom as well as all fractions. Components of venom and fraction with molecular weight above 30kDa were more intensely recognized by ASV, but low molecular weight components in venom and fraction 1,2,3,4 were weakly recognized by western blot analysis. Therefore, it was concluded that cobra venom had various enzymatic activities and low immunogenicity in low molecular weight components of venom can also be seen by western blot pattern. Result of present study fulfill the lack of scientific information regarding the components of cobra venom in Myanmar which is lethally toxic and provide potential benefit for development of cobra specific anti-venom by advanced molecular technology. By knowing the constituents of cobra venom proteins can be useful for explaining clinical effects of snake bite victims.
IRB/PRC/ERC Approval Date : 2016-07-01
Placement of IRB/PRC/ERC : Department of Medical Research
IRB/PRC/ERC Approval Letter/Document : Cobra.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -