Health Research Registry List

Research Registry No : HRID-00104_V7
 
University Departmental Research :
 
Registration Date : 2019-05-30
 
Title of Research : Characterization of Hyaluronidase isolated from Russell’s Viper (Daboia siamensis) Venom of Myanmar
 
Principle Investigator : Thet Thet Mar
 
Co-authors : Win Aung, Zaw Myint, Lwin Zar Maw, Tin Ko Ko Oo, Nwe Ni Aung
 
Field of Research : Biochemistry
 
Publication Source : Myanmar Health Research Congress. 2016
 
 
Year of Publication : 2016
 
URL of Publication : www.mhrc-mohs.com
 
Presentation Source : 44th Myanmar Health Research Congress. 2016; P67
 
Placement of Presentation : Department of Medical Research, Yangon, Myanmar
 
Year of Presentation : 2016
 
Abstract : The spreading factor, hyaluronidase enzyme is one of the components of snake venom which contributes to local damage at bite site by affecting the extracellular matrix and potentiates the toxicity of venom. Hyaluronidase cleaves the major component of extracellular matrix (ECM) of vertebrates’ hyaluronic acid (HA), which connects protein filaments, collagen fibers and the connective tissue cells. It increases tissue permeability and enhances the rate of venom absorption. This study was conducted with an aim to assess the characterization of hyaluronidase enzyme isolated from Russell’s Viper (Daboia siamensis) Venom (RVV) of Myanmar. Firstly, hyaluronidase enzyme was isolated from crude RVV by using HiTrap SP FF strong cation exchange chromatography. The chromatographic profile of RVV showed 8 different major peaks. Hyaluronidase activity was then determined by Pukrittayakamee method. Hyaluronidase enzyme activities were detected only at peak 6 and 7. The turbidity reduction units of crude RVV, peak 6 and 7 were found to be 47.43, 25.17 and 25.48 respectively. By using ELISA method, it was noted that the specific monovalent anti snake venom (ASV) did not neutralize the hyaluronidase enzyme activity. It was also confirmed by Western blot analysis in which the ASV could not totally recognize the hyaluronidase enzyme. Therefore, it could be concluded that anti-hyaluronidase activity was not detected in locally produced ASV. It may be due to lack of heat- labile hyaluronidase enzyme in crude venom used in immunization of horse or less immunogenic property of enzyme itself. It is suggested that improvement of methodology for production of potent anti-snake venom needs to be considered for achieving a more potent ASV, leading to reduction of morbidity and mortality resulted from snake bite in our country.
 
 
IRB/PRC/ERC Approval Date : 2015-12-28
 
Placement of IRB/PRC/ERC : Department of Medical Research
 
IRB/PRC/ERC Approval Letter/Document : Hya.pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Website : -