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Research Registry No |
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HRID-00120_V4 |
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University Departmental Research |
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Registration Date |
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2019-05-21 |
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Title of Research |
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Establishment of molecular strain typing methods for Mycobacterium tuberculosis in Myanmar |
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Principle Investigator |
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WAH WAH AUNG |
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Co-authors |
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Phyu Win Ei, Mi Mi Htwe, Wint Wint Nyunt, Thyn Lei Swe, Thandar Lwin, Jong Seok Lee, Chulhum L Chang, Si Thu Aung |
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Field of Research |
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Bacteriology |
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Publication Source |
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Southeast Asian Journal of Tropical Medicine and Public Health 2018, 49(22): 276-284 |
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Year of Publication |
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2018 |
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URL of Publication |
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http://www.tm.mahidol.ac.th/seameo/2018-49-2/10-7351-4-276.pdf |
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Presentation Source |
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48th UNION World Conference on Tuberculosis and Lung Diseases Conference, Poster Presentation |
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Placement of Presentation |
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Guadalajara, Mexico |
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Year of Presentation |
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2017 |
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Abstract |
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. Mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing is a fast and promising method to discriminate Mycobacterium tuberculosis (MTB) strains. The present study was carried out to evaluate the applicability of MIRU-VNTR method for genotyping of clinical MTB strains from new pulmonary TB patients in Myanmar, and to compare the discriminatory ability between 15 loci and 24 loci MIRU-VNTR typing. One hundred and seven clinical MTB isolates which were collected during 2012-2013 were examined for anti-TB drug susceptibility using both proportion method and commercial line-probe assay. The results of internationally standardized 15 loci and 24 loci MIRU-VNTR typing methods were analyzed by a MIRU-VNTR plus web application together with Hunter-Gaston discriminatory index (HGDI) as numerical index to describe the discriminatory power. All tested MTB isolates showed unique patterns, which did not cluster and were distributed among 14 lineages. EAI genotype (30%) was the most prevalent genotype, followed by Bei-jing genotype (29%) that is significantly associated with multidrug-resistant TB (p<0.0004). Both 15 and 24 loci MIRU-VNTR were highly discriminatory (HGDI= 0.9833 and 0.9874, respectively). This study indicates that MIRU-VNTR could be used as a genotyping tool for Myanmar MTB strains |
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IRB/PRC/ERC Approval Date |
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2012-12-13 |
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Placement of IRB/PRC/ERC |
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Department of medical Research |
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IRB/PRC/ERC Approval Letter/Document |
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Dr WWA_Ethical Molecular typing KOICA_2012.pdf
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Pre-existing Registration ID |
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- |
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Pre-existing Name of Organization |
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- |
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Pre-existing Website |
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