Health Research Registry List

Research Registry No : HRID-00184_V8
University Departmental Research :
Registration Date : 2019-05-17
Title of Research : Phylogenetic Analysis of Myanmar Human Respiratory Syncytial Virus from Under Five Children with Acute Respiratory Infection Admitted to Yangon Children Hospital
Principle Investigator : Kay Thi Aye
Co-authors : Lay Myint Yoshida, Aung Zaw Latt, Hlaing Myat Thu, Theingi Win Myat, Hsu Htet Thwe, Sandar Aung, Ye
Field of Research : Virology
Publication Source : Myanmar Health Sciences Research Journal. 2018; 30(2): P146-154
Year of Publication : 2018
URL of Publication : -
Presentation Source : 45th Myanmar Health Research Congress. 2017; P48
Placement of Presentation : Department of Medical Research, Yangon
Year of Presentation : 2017
Abstract : Human respiratory syncytial virus (RSV) is one of the most important respiratory viruses responsible for acute respiratory tract infection (ARI) outbreaks in children worldwide. RSV is a member of the family Paramyxoviridae in which differentiated into two groups (A and B) based on antigenic and genetic variability. To date, 11 genotypes for RSV group A and 23 for RSV group B have been described based on changes in the G gene coding for the attachment glycoprotein. In this study, nasopharyngeal swab samples were collected from hospitalized pediatric ARI cases at Yangon Children Hospital from January to September, 2014. Of 160 cases, non-structural protein 1 (NS1) gene of RSV was detected in 16.3% (26/160), comprising RSV-A strains 52% (11/21) and RSV- B strains 48% (10/21). Furthermore, 21 NS1 gene-positive nasopharyngeal swab samples were processed for genotyping by sequencing of C terminal of the G gene, second variable region. G gene of the RSV was successfully sequenced in 61.9% (13/21) of samples. RSV-A strain was the larger group, accounting for 53.8% (7/13), followed by RSV-B, 38.5% (5/13) and one case 7.7% (1/13) was a mixed infection. The phylogenetic analysis revealed that all group-A strains clustered as the ON1 genotype. Additionally, 72 nucleotide duplication in the second highly variable region of attachment G gene was observed in all RSV On1 genotype in subgroup-A isolates. Moreover, one isolated of ON1genotype showed G284S substitution as a novel mutation. Molecular surveillance of RSV infection should be conducted in Myanmar.
IRB/PRC/ERC Approval Date : 2015-08-17
Placement of IRB/PRC/ERC : Department of Medical Research, Yangon
IRB/PRC/ERC Approval Letter/Document : Dkta.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -