Health Research Registry List

Research Registry No : HRID-00185_V2
 
University Departmental Research :
 
Registration Date : 2019-05-17
 
Title of Research : Detection of extensively drug resistant tuberculosis (XDR-TB) among multidrug resistant Mycobacterium tuberculosis clinical isolates from two TB centers in Myanmar
 
Principle Investigator : Phyu Win Ei
 
Co-authors : Wah Wah Aung, Wint Wint Nyunt, Thyn Le Swe, Mi Mi Htwe, Su Mon Win, Chulhun L Chang, Hyeyoung Lee, Jong Seok Lee
 
Field of Research : Bacteriology
 
Publication Source : International Journal of Tuberculosis and Lung Disease, 22(1), 47-53.
 
 
Year of Publication : 2018
 
URL of Publication : https://doi.org/10.5588/ijtld.17.0321
 
Presentation Source : International Meeting of the Federation of Korean Microbiological Societies. 2017
 
Placement of Presentation : Korea
 
Year of Presentation : 2017
 
Abstract : Setting: Two tuberculosis (TB) reference laboratories in Myanmar. Objectives: To determine the proportion of extensively drug-resistant TB (XDR-TB) cases among multidrug-resistant TB (MDR-TB) cases and the mutations that cause resistance to second-line drugs in Myanmar. Design: This was a cross-sectional, retrospective study. Multidrug-resistant Mycobacterium tuberculosis isolates were collected during 2015-2016. Phenotypic drug susceptibility testing (DST) was performed and drugresistant mutations identified by sequencing. Genotypes were determined to explain relationships between drug resistance patterns and genotypes. Rwsults: Of 89 MDR-TB isolates, 12 were XDR-TB and 24 were pre-XDR-TB, with 21 resistant to fluoroquinolones (FQs) and 3 to second-line injectable agents (SLIDs). High rates of cross-resistance among second-line drugs were observed. Correlations between phenotypic and molecular DST against FQs and SLIDs were 91% in both cases. The most frequent mutation in FQ-resistant isolates was D94G (8/21) in gyrA and A1401G (11/15) in rrs in those resistant to SLIDs. The dominant genotype was the Beijing type (76/89). Conclusion: There were high proportions of XDRTB and pre-XDR-TB among MDR-TB cases; crossresistance among second-line drugs was high, with various types of genetic mutations. These data suggest that resistance to second-line anti-tuberculosis drugs should be monitored intensively, and molecular DST should be employed.
 
 
IRB/PRC/ERC Approval Date : 2016-08-05
 
Placement of IRB/PRC/ERC : Department of Medical Research, Yangon
 
IRB/PRC/ERC Approval Letter/Document : phyu XDR ethical.pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Website : -