Health Research Registry List

Research Registry No : HRID-00347_V4
University Departmental Research :
Registration Date : 2019-05-17
Title of Research : Anti-inflammatory effect of Vitex negundo Linn. ( ကြောင်ပန်းကြီး ) leaves and its Chemical Composition by GC-MS
Principle Investigator : Ei Ei Htway
Co-authors : Aye Aye Phyu, Khin May Thi, Kyaw Min Aung, Aye Aye Tun, Swe Zin Aung, Thiri Hlaing, Sandar Lin, Maung Maung Thet
Field of Research : Pharmacology
Publication Source :
Year of Publication : -
URL of Publication : -
Presentation Source : 47th Myanmar Health Research Congress, 2019; P 73
Placement of Presentation : Department of Medical Research
Year of Presentation : 2019
Abstract : Vitex Negundo Linn. (ကြောင်ပန်းကြီး) is a useful Myanmar medicinal plant and traditionally used with the purposes to treat rheumatism, muscular pain and muscle cramps. Plants are good sources of biologically active compounds and some of the western medicines that are used to treat inflammation are also the plant derivatives. This study evaluated the in-vivo anti-inflammatory activity of 70% ethanolic extract of Vitex negundo Linn. (Vn) leaves and investigated the bioactive constituents of leaf extract by Gas Chromatography-Mass Spectrometry (GC-MS). In evaluation of the anti-inflammatory effect, the response of reduction in carrageenan-induced paw oedema in albino rat was measured by plethysmometer. A total of five groups (n=8) were tested, one negative control group (vehicle 10ml/kg), one positive control group (aspirin 300mg/kg) and three tested groups (Vn extract 250, 500 and 1000mg/kg) respectively. Among the three tested groups, leaves extract 250 mg/kg and 500 mg/kg single oral doses could not inhibit in carrageenan- induced paw oedema in rats. However, the leaves extract 1000 mg/kg inhibited the carrageenan- induced paw oedema at 2 hour (p = 0.050) compared to control group. Acute toxicity of 70% ethanolic extract of Vn was also done by the OECD 425 guideline on albino rat and the study shown that its LD50 value was greater than 5000 mg/kg. The bioactive phytoconstituents of the leaf extract were identified by mass spectral data of National Institute of Standard and Technology (NIST) libraries. The bioactive constituents of leaf extract were 9-octadecenoic acid (30.30%), ascorbic acid dihexadecanoate (8.72%), hydroxymethyl furancaroxaldehyde (4.13%), 1-alanine ethylamide (2.61%), 9-octadecanoic acid propane triylester (2.55%), olealdehyde (2.00 %), dihydroxy propyl elaidate (1.78%), quinic acid (1.45%), dihydro maltol derivative (1.16%) and phytol (1.00%) and other fifteen minor compounds were also found out. Most of the major constituents of the 70% ethanolic extract were anti-oxidant compounds and compounds given anti-inflammatory activity were present only in the minor constituents. The results from this study indicated that 70% ethanolic extract of Vn leaves is composed of medicinally useful bioactive compounds. But it did not find significant anti-inflammatory action at the tested doses.
IRB/PRC/ERC Approval Date : 2017-09-29
Placement of IRB/PRC/ERC : DMR(POLB)
IRB/PRC/ERC Approval Letter/Document : KPG approve.docx
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -