Health Research Registry List

Research Registry No : HRID-00385_V3
University Departmental Research :
Registration Date : 2019-05-17
Title of Research : Determination of anti-malarial efficacy of various fractions of Ocimum sanctum Linn. (Pin-sein-net) leaf on Plasmodium berghei infected mice.
Principle Investigator : Mu Mu Sein Myint
Co-authors : Kyin Hla Aye, Khin Tar Yar Myint, Ye Htut, May Aye Than, Ei Ei Soe, Aung Aung Maw and Mi Aye Aye Mon
Field of Research : Pharmacology
Publication Source :
Year of Publication : -
URL of Publication : -
Presentation Source : 41st Myanmar Health Research Congress, Programme and Abstracts;. 2013:P 31
Placement of Presentation : Department of Medical Research
Year of Presentation : 2013
Abstract : The aim of this study is to evaluate the antimalarial effect of petroleum ether, ethanol and watery fractions of Ocimum sanctum Linn. (Pin –sein-net) in –vivo Plasmodium berghei infected mice model. Yield percent of successive fractionations of O.sanctum leaf by using soxhlet apparatus were p-ether soluble fraction 3.6% , ethanol soluble fraction 17.1% and water soluble fraction 16.3% respectively. Phytochemical test and acute toxicity test of these three fraction were also done. In phytochemical test, alkaloid, flavonoid, polyphenol, amino acid and tannin were detected in ethanol and watery fractions, steroid/ terpene was only detected in petroleum ether and ethanol fractions. Glycoside was detected in all three fraction. Acute toxicity test was done according to the method of Litchfield &Wilcoxin. Toxic effect was not found in all three fractions at the maximum permissible dose. Therefore the medium lethal dose (LD 50 value) was more than 9gm/kg body weight in p-ether fraction and 12gm/kg body weight in ethanol and watery fractions. Suppressive test for antimalarial effect on P. berghei infected mice model was done according to the method of Peter. The results were expressed in terms of percent parasite suppression on the day 4. Of the three fraction, percent parasite suppression of ethanol fraction of 3gm/kg body weight, 6gm/kg body weight and 9gm/kg body weight showed the highest suppressive effect, 36.62%, 40.14% and 41.54% respectively. The present study showed that ethanol fraction of Ocimum sanctum leaves had significant antimalarial effect.
IRB/PRC/ERC Approval Date : 2010-09-10
Placement of IRB/PRC/ERC : Department of Medical Research
IRB/PRC/ERC Approval Letter/Document : PE 1.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -