Health Research Registry List

Research Registry No : HRID-00390_V1
 
University Departmental Research :
 
Registration Date : 2019-05-17
 
Title of Research : Acute Toxicity of Three Medicinal Plants Using as Antidote in Myanmar
 
Principle Investigator :
 
Co-authors : Khin May Thi, Ei Ei Htway, Aye Min Maw, Moh Moh Lwin, Aye Thida Htun, Zin Nwe Soe, Rai Kit
 
Field of Research : Pharmacology
 
Publication Source :
 
 
Year of Publication : -
 
URL of Publication : -
 
Presentation Source : The 9th traditional medicine Research Congress, 2019, p-23
 
Placement of Presentation : University of Traditional Medicine, Mandalay
 
Year of Presentation : 2019
 
Abstract : Snake bite and scorpion sting are the important causes of morbidity (life-threatening) and mortality for human being of rural area. In Myanmar, more than 8000 cases are reported and an estimated mortality rate was 750 to 1000 die annually each year. Regarding snake bite registry, Mandalay region was more than Ayeyarwady (1203 vs 122). In Myanmar Traditional Medicine and international literature, Stachytarpheta indica (L.) Vahl (အဆိပ်တစ်ရာ), Mimosa Pudica L. (ထိကရုန်း) and Amaranthus spinosus L. (ဟင်းနုနယ်ဆူးပေါက်), have been used in blood disease, asthma, fever, uterine complaints, haemotometra, inflammation, edema, diuretic, urinary complaints and also as antidote for snake, scorpion, insect stinging evenomation. The present study was aimed to determine the acute toxicity of these three medicinal plants which were used as antidote in Myanmar. Different extracts of aerial part of Stachytarpheta indica (L.)Vahl, the whole plant of Mimosa pudica L. and root of Amaranthus spinosus L. were prepared by Soxhlet extraction method with ethanol (95%). Acute oral toxicity testing on rats was performed by the main test according to OECD 425 guideline, (2008). The up and down procedure would be adopted based on toxic system of each dose according to guideline. In phytochemical screening, all three plants showed the presence of alkaloids, tannins, phenolic compounds, steroids, carbohydrates, glycosides and saponin. Protein and cyanogenic glycoside were not detected. The phytochemical compound, flavonoids was detected only Mimosa pudica L. and Stachytarpheta indica (L.)Vahl. From acute toxicity study, a single5000mg/kg dose of ethanol extracts did not show any lethal effect on the tested animals. The LD50 of the three plants were more than 5000mg/kg and it was calculated by AOT425 StatPgm software. Therefore the present study proved that these three medicinal plants had no serious acute toxic effects. This study can give toxicity data and provide safety information for the practitioners and consumers.
 
 
IRB/PRC/ERC Approval Date : 2017-11-10
 
Placement of IRB/PRC/ERC : Department of Medical Research (Pyin Oo Lwin Branch)
 
IRB/PRC/ERC Approval Letter/Document : Daw SZA.docx
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Website : -