Health Research Registry List

Research Registry No : HRID-00393_V6
 
University Departmental Research :
 
Registration Date : 2019-05-17
 
Title of Research : Role of flow cytometric immunophenotyping in diagnosis of multiple myeloma
 
Principle Investigator : San San Htwe
 
Co-authors : Htun Lwin Nyein , Aye Mya Khine, Khin La Pyae Tun, Win Pa Pa Naing, Ni Ni Win, Moe Thuzar Min , Sein Win, Khin Saw Aye
 
Field of Research : Others
 
Publication Source : Myanmar Health Sciences Research Journal. 2018; 30(1): P52-57
 
 
Year of Publication : 2018
 
URL of Publication : www.myanmarhsrj.com
 
Presentation Source : 45th Myanmar Health Research Congress. 2017; P42)
 
Placement of Presentation : Department of Medical Research
 
Year of Presentation : 2017
 
Abstract : Immunophenotyping has become an invaluable tool in the management of hematological malignancies and is increasingly finding a role in the diagnosis and monitoring of plasma cell disorders. The aim of the study was to provide an accurate diagnosis of multiple myeloma by application of flow cytometry immunophenotyping. A cross sectional descriptive study was conducted at Department of Clinical Hematology, Yangon General Hospital from September 2015 to August 2016. Multiparametric flow cytometry immunophenotyping was performed using monoclonal antibodies against CD56, CD19, CD138, CD38 and CD45. A total of 40 clinically suspected cases of multiple myeloma were included for the study. Among the 40 cases, 25 cases (62.5%) were diagnosed as multiple myeloma by the positive expression of CD138 or CD38, negative CD19 expression, weak or negative CD45 expression and positive or negative CD56 expression. Age of the patients ranged from 49 years to 89 years. The male to female ratio was 1.1:1. Serum protein electrophoresis and densitometer reading were performed in all cases for detection of monoclonal band and monoclonal protein concentration. Monoclonal band was detected visually and estimation of monoclonal protein was done by densitometer. Among the 40 cases, 25 cases (62.5%) had monoclonal gammopathy. Among these cases, 20 cases (80%) had monoclonal band in the gamma () region and 5 cases (20%) had in the beta () globulin region. The mean concentration of monoclonal protein was 4.50 g/dl, with a range of 2.28 to 7.95 g/dl. The remaining 15 cases (37.5%) were not detected monoclonal band. In conclusion, flow cytometry immunophenotyping is useful tool for the diagnosis of multiple myeloma and it should be included as a routine assay in monoclonal gammopathy patients.
 
 
IRB/PRC/ERC Approval Date : 2015-01-21
 
Placement of IRB/PRC/ERC : Department of Medical Research
 
IRB/PRC/ERC Approval Letter/Document : MMFCM_ERC.pdf
 
Pre-existing Registration ID : -
 
Pre-existing Name of Organization : -
 
Pre-existing Website : -