|
Abstract |
: |
Aegle marmelos L. Correa belongs to family Rutaceae and is abundantly available in Myanmar. It is popularly known as Bael tree, Ok-shit in Myanmar. The aim of current research was to analyse chemical compositions, antioxidant activity, total phenolic content and acute toxicity study of leaves of Aegle marmelos L. Correa (Ok-shit). Phytochemical constituents present in Ok-shit leaves were carried out Raaman (2006). The chemical compositions were studied by Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared spectrophotometer (FTIR). The antioxidant activity was studied qualitatively by DPPH-TLC bioautography method and quantitatively by DPPH radical scavenging assay. The total phenolic content was measured by Folin Ciocalteu method using UV- Visible Spectrophotometer. Acute toxicity was done OECD 425 guideline (2008). In phytochemical analysis, Ok-shit leaves contained alkaloids, carbohydrates, flavonoids, glycosides, phenols, protein, reducing sugar, saponins, tannins and tri-terpene. According to GC-MS analysis, eight bioactive compounds were identified, which possess many medicinal properties. Among them, α- pinene, caryophyllene, β-copaene, germacrene and 9-octadecenoic acid (Z) -methyl ester have antioxidant activity. The FTIR analysis observed the presence of hydroxyl group, sp2 hydrocarbon, sp3 hydrocarbon, carbonyl group, C=C stretching vibration and C-C-O stretching vibration. In TLC- bioautography, the bands with Rf value of 0.75 and 0.85 showed antioxidant activity. The antioxidant activity of Ok-shit leaves and standard ascorbic acid were IC50 values 49.7 µg/mL and 2.7µg/mL. Total phenolic content was 47 GAEs/g. Acute toxicity study revealed that, there was no toxic sign and lethality at the dose of 5000 mg/kg (LD50>5000 mg/kg). The results of current study indicated that, Ok-shit leaves contained various bioactive compounds and gave scientific support in traditional medicine. And also recommended as a plant of phytopharmaceutical importance and safe for consumption.
Key Words: GC-MS, FTIR, UV-Visible Spectrophotometer, Antioxidant, Aegle marmelos
|
