Health Research Registry List

Research Registry No : HRID-00411_V1
University Departmental Research :
Registration Date : 2019-05-22
Title of Research : Diagnosis of extra pulmonary tuberculosis of clinically suspected cases by PCR
Principle Investigator : Kyi May Htwe
Co-authors : Aye Aye Lwin, Khine Zar Win, Hlaing Mya Win, Khin Saw Aye
Field of Research : Bacteriology
Publication Source : -
Year of Publication : -
URL of Publication : -
Presentation Source : 46th Myanmar Health Research Congress. 2017; P158, poster 69
Placement of Presentation : Department of Medical Research
Year of Presentation : 2017
Abstract : Tuberculosis (TB) is one of the major public health problems and caused by various species of mycobacteria among which Mycobacterium Tuberculosis (MTB) is the most frequent specie. Extra-pulmonary tuberculosis (EPTB) constitutes about 15-20% of TB cases. This study was conducted to diagnose EPTB by Polymerase Chain Reaction (PCR) as an additional tool along with conventional method which is AFB smear by Ziehl-Neelsen staining. A total of 109 clinically suspected tuberculosis patients attending Medical Unit 1,Yangon General Hospital from 2016 to 2017 were included in this study. All patients were AFB smear negative and three different types of samples were analysed for MTB. Mycobacterial DNA was extracted from 47 samples (43.1%) of cerebrospinal fluid, 32 samples (29.4%) of pleural fluid, 29 samples (26.6%) of ascitic fluid and 1 sample (0.9%) of pericardial fluid using beads solution method. PCR amplification was performed to detect a 123-bp region from MTB targeting the IS6110. Mean age of study population was 44.7 ± 16.8 and age ranged from 13 to 78 years. About 55.04% (60/109) of male gender were included in this study. 16.5% (18/109) of patients had previous history of TB, 9.2% (10/109) got anti-TB treatment and 10.1% (11/109) had history of TB contact in family. Most of patients (70%) had low grade fever and weight loss. About 3.7% (4/109samples) were positive by PCR for MTB and those were three pleural fluid and one ascetic fluid sample. All PCR positive patients were male. In suspected EPTB cases, when conventional microbiological tests almost fail, PCR results along with the clinical presentation may be adequate to initiate anti-tuberculous treatment.
IRB/PRC/ERC Approval Date : 2016-10-07
Placement of IRB/PRC/ERC : Department of Medical Research
IRB/PRC/ERC Approval Letter/Document : DKMH.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -