Health Research Registry List

Research Registry No : HRID-00422_V1
University Departmental Research :
Registration Date : 2019-05-23
Title of Research : Detection of Enterotoxigenic and methicillin resistant Staphylococcus aureus in ice cream
Principle Investigator : Su Nyein Aye
Co-authors : Dr Mo Mo Win, Dr Kyi Kyi Nyein Win, Dr Moh Moh Hlaing, Daw Thin Thin Wah, Daw Tin Tin Htwe, Daw May Than Htay, Daw Zin Mi Thein, Daw Nilar
Field of Research : Bacteriology
Publication Source : Pending
Year of Publication : -
URL of Publication : -
Presentation Source : 47th Myanmar Health Research Congress.2019; P149
Placement of Presentation : Department of Medical Research
Year of Presentation : 2019
Abstract : The study for detection of enterotoxigenic and methicillin resistant staphylococcus aureus in ice cream was conducted from Oct 2017 to Sep 2018. The study aims to detect the enterotoxigenic and methicillin resistant staphylococcus aureus in ice cream from street vendors in Yangon. Dairy products are common sources of Staphylococcus aureus infection in food poisoning if Staphylococcus aureus has ability to produce enterotoxin. Staphylococcus aureus is the most important pathogen in food borne intoxication and it has recently emerged as an important cause of community-associated staphylococcal infections. Methicillin resistant staphylococcus aureus (MRSA) can be transferred to humans through the consumption, handling, and spillage of unpasteurized milk and dairy production. In this study, a total 87 ice cream samples were collected from street vendors in Yangon. Samples were collected by sterile container, transported by ice box and analysed at the laboratory. One milliliter of ice cream sample was placed in a sterile tube containing 9mlof sterile peptone water and homogenized using a vortex for 10 to 15 sec. After homogenization, the mixtures were spread over the selective medium, Baird-Parker agar. Then, it was incubated aerobically at 37 °C for 24 hr. Typical coagulase-positive Staphylococcus aureus colonies are dark grey to black and are surrounded by an opaque halo of precipitation which signified the coagulase reaction and colonies were further identified by gram stain, catalase test, anaerobic utilization of glucose, manitol, and coagulase test. Antimicrobial susceptibility testing of the isolates was performed on Mueller–Hinton agar by the disk diffusion method using commercial antibiotic discs in accordance with CLSI guidelines 2016. Staphylococcus aureus enterotoxin was detected by SET RPLA method. In this study, coagulase positive Staphylococcus aureus was isolated in 20 samples of ice cream (22.9%). Among then, 2 samples (2/20) (10%) were identified as MRSA. Moreover, 8 out of 20 (40%) Staphylococcus aureus produced enterotoxins. Among them, (35 %) 7 was staphylococcal enterotoxin C and (5 %) 1 was staphylococcal enterotoxin B respectively. Staphylococcal enterotoxins are cause of concern for community health so that the findings from this study will contribute the beneficial support for food safety program.
IRB/PRC/ERC Approval Date : 2017-11-16
Placement of IRB/PRC/ERC : Department of Medical Research
IRB/PRC/ERC Approval Letter/Document : SNA ethic ice cream.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -