|
Research Registry No |
: |
HRID-00637_V1 |
| |
|
University Departmental Research |
: |
Yes |
| |
|
Name of University |
: |
University of Medical Technology, Yangon |
| |
|
Department |
: |
Medical Laboratory Technology |
| |
|
Registration Date |
: |
2020-03-14 |
| |
|
Title of Research |
: |
Detection of Rh DEL phenotype by using adsorption elution method and its association with other Rh antigens among Rh negative blood donors |
| |
|
Principle Investigator |
: |
Saw Thu Wah |
| |
|
Co-authors |
: |
Daw Saung Nay Chi, Daw Kyi Kyi Kyaing, Prof. Dr Aye Aye Khin, Dr. Thida Aung |
| |
|
Field of Research |
: |
Clinical Haematology |
| |
|
Publication Source |
: |
Advances in Hematology. 2020;2020 |
| |
| |
|
Year of Publication |
: |
2020 |
| |
|
URL of Publication |
: |
- |
| |
|
Presentation Source |
: |
|
| |
|
Placement of Presentation |
: |
Hindawi |
| |
|
Year of Presentation |
: |
2020 |
| |
|
Abstract |
: |
Background. Red cell Rhesus (Rh) antigen expression is influenced by the genetic polymorphism of RHD and RHCE genes and
reveals serologically different reactions of RhD variants such as partial D, weak D, and Rh-Del. Serologically, Rh-Del type can only
be detected by an adsorption-elution technique, and it might be mistyped as Rh-negative. e prevalence of Rh-Del has not been
reported yet in Myanmar. Method. A total of 222 Rh-negative blood donors in the National Blood Center were tested for weak D
and Rh-Del by indirect anti-human globulin and adsorption-elution method, respectively. RhCE typing was performed among Rh-negative and Rh-Del. Results. Of them, 75.2% (167/222) were Rh-negative, 15.8% (35/222) were Rh-Del, and 9% (20/222) were
weak D. Of 202 blood donors (167 true Rh-negative and 35 Rh-Del), all of the Rh-Del positives were C-antigen-positive with 94.3%
Ccee phenotype (33/35) and 5.7% CCee (2/35). Most of the Rh-negative donors (80.2%) were ccee phenotype (134/167).
Conclusion. About half of Rh-Del subjects were repeated donors, and attention was needed to avoid transfusion of truly Rh-negative patients to prevent alloimmunization. It is recommended to do Rh-Del typing of Rh-negative donors who are C-antigen positive and consider moving them to the Rh-positive pool. Further study is needed to clarify the alloimmunization status for
transfusion of Rh-Del blood to Rh-negative recipients. Molecular markers for RhD-negative and D variants should be established
in the Myanmar population to improve the selection of antisera for Rh typing and enhance the safety of the transfusion services. |
| |
| |
|
IRB/PRC/ERC Approval Date |
: |
2018-04-04 |
| |
|
Placement of IRB/PRC/ERC |
: |
University of Medical Technology |
| |
|
IRB/PRC/ERC Approval Letter/Document |
: |
14032020100759IRBcertificate(DEL).pdf
|
| |
|
Pre-existing Registration ID |
: |
- |
| |
|
Pre-existing Name of Organization |
: |
- |
| |
|
Pre-existing Website |
: |
- |
