Health Research Registry List

Research Registry No : HRID-00659_V1
University Departmental Research : No
Registration Date : 2020-02-27
Title of Research : Environmental Study on Carbapenem-Resistant Enterobacteriaceae in drinking water and waste water in selected township of Yangon, Myanmar
Principle Investigator : Thuzar Myint
Co-authors : Dr.htdeharu Hagiya, Dr. Mya Mya Aye
Field of Research : Microbiology
Publication Source : no
Year of Publication : 2018
URL of Publication : -
Presentation Source : 46 th Myanmar Health Research Congress. 2018; P 139-140
Placement of Presentation : DMR
Year of Presentation : 2018
Abstract : Carbapenem-resistant Enterobacteriaceae (CRE) is Gram negative bacteria that are difficult to treat because of the highly resistance to antibiotics and an emerging problem of this century. Preventing the transmission of CRE is a public health priority. A cross-sectional descriptive study was carried out to detect CRE in drinking and waste water samples in Latha and North Okkalapa townships in Yangon from December 2016 to November 2017. Drinking water samples were collected from currently using containers and waste water samples were collected from street drains of the selected households. A total of 220 samples (108 from Latha and 112 from North Okkalapa townships, respectively) were aseptically collected in sterile 50 mL tubes and transported to Bacteriology Research Division. After concentration by centrifugation, each sample was inoculated onto M-ECC: CHROM agar containing 0.25 μg/mL meropenem and incubated at 37°C overnight. Then the microbial organism identification and antimicrobial susceptibility testing were carried out by using the Vitek 2 automated system and carbapenemase genes were detected by Polymerase Chain Reaction (PCR). CRE were not detected in all drinking water samples from both townships. A total of 181 bacterial isolates were detected in 54 waste water samples from Latha Township. Of these isolates, CRE was detected in 8.8% (16/181); Escherichia coli 31.2% (5/16), Citrobacterfreundii 25% (4/16) and Enterobacteras buriae 25% (4/16), and Klebsiella pneumoniae 18.8% (3/16). A total of 305 bacterial isolates were detected in 56 waste water samples from North Okkalapa Township. Of these isolates, CRE were detected in 11.1% (34/305); E. coli 52.9% (18/34), K.pneumoniae 17.6% (6/34), E. clocacae 23.5% (8/34) and Providencia spp 5.9% (2/34). Among the CRE isolates, molecular analysis based on PCR revealed that 12 NDM genes from Latha township (25%), and 28 NDM genes (82.4%) and 1 NDM & OXA-48 genes (2.9%) from North Okkalapa Township. CRE positive waste water sampling sites were found in 16.7% (9/54) in Latha and 21.4% (12/56) in North Okkalapa townships. This study demonstrated the current distribution of CRE in environments in Yangon and is of great help in grasping the real spread of CRE in Myanmar.
IRB/PRC/ERC Approval Date : 2017-01-02
Placement of IRB/PRC/ERC : dmr
IRB/PRC/ERC Approval Letter/Document : 27022020080702Dawthuzarmyint_20200227_0001.pdf
Pre-existing Registration ID : -
Pre-existing Name of Organization : -
Pre-existing Website : -